Yonsei Advanced Imaging Center
in cooperation with Carl Zeiss Microscopy

기기설명 :

Feature

LSM 900은 초고감도 GaAsP-PMT detector를 탑재하여 매우 약한 시그널도 선명하게 이미징 할 수 있으며, crosstalk 현상이 없이 multi-spectral imaging이 가능한 최첨단 컨포칼 현미경입니다. 특히LSM 900는 Aryscan2를 장착하여, 기존 컨포컬 현미경 관찰에 사용하는 시료를 SR mode로 촬영하면, 컨포칼 현미경보다 1.7배 향상된 초고해상도 이미지를 획득할 수 있습니다.

• 최대 해상도: Lateral: 120 nm, Axial: 350 nm (Airyscan2 SR mode)
• 속도: 18.9 frames per second at 512 X 512 pixels

Application

• 초고해상도 Large volume Imaging: 3D cells, Organoids, Tissue Section, Cleared sample, Model Organisms, Plant roots
• 초고해상도 Live-cell Imaging: Whole living embryo, Multi-labeled living cells, FRET, FRAP, bleaching experiment
• Overlapping emission signals, More than 4ch simultaneous multi-spectral imaging

사양 :

1. Microscopy
   • Mode : Conventional mode, Laser mode : Brightfield, DIC, Fluorescence, Confocal, Super-Resolution
   • Type : Inverted: Axio Observer 7
2. Scan module
   • Scan resolution : 4 × 1 to 6144 × 6144 pixels
   • Scan rotation : Free rotation (360 degrees), in steps of 1 degree variable; free xy offset
   • Detectors : 3ch GaAsP PMT, 1 PMT Transmit detector
3. Lasers
   405, 488, 561, 640 nm
4. Objective
   • Plan-Apochromat 5x/0.16
   • Plan-Apochromat 10x/0.45
   • Plan-Apochromat 20x/0.8
   • C-Apochromat 40x/1.2 W
   • Plan-Apochromat 63x/1.40 Oil
5. Software
   ZEN 3.5
6. Incubation
   Stage Top type, Temperature, CO2, heating control on confocal software